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anti ci m6pr  (Proteintech)


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    Structured Review

    Proteintech anti ci m6pr
    Anti Ci M6pr, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+igf2r/IGF2R-Specific+Antibody/pmc12802303-207-42-50
    Average 94 stars, based on 39 article reviews
    anti ci m6pr - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Combining light-induced aggregation and biotin proximity labeling strongly implicates endolysosomal proteins in early α-synuclein oligomerization
    Article Snippet: .. Membranes were then first incubated with primary antibodies for 1h: anti-mCherry (AB167453 Abcam), anti-actin (G043 ABM), anti-IGF2R (20253-1-AP Proteintech), anti-BLTP3A (25121-1- AP Proteintech), anti-WDR44 (A301-440A Thermofisher) and anti-PRDX6 (13585-1-AP Proteintech). .. The membranes were then washed 3 times 10 min with PBS-Tween 0.1% prior incubation with secondary antibodies matching the host of the primary antibodies using either 680RD or 800CW LiCor antibodies (LiCor, cat. 926-68071).

    Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization
    Article Snippet: .. Membranes were then first incubated with primary antibodies for 1h: anti-mCherry ( AB167453 Abcam), anti-actin (G043 ABM), anti-IGF2R (20253-1-AP Proteintech), anti-BLTP3A (25121-1-AP Proteintech), anti-WDR44 (A301-440A Thermofisher) and anti-PRDX6 (13585-1-AP Proteintech). .. The membranes were then washed 3 times 10 min with PBS-Tween 0.1% prior incubation with secondary antibodies matching the host of the primary antibodies using either 680RD or 800CW LiCor antibodies (LiCor, cat. 926–68071).

    Article Title: CD34 + CLDN5 + tumor associated senescent endothelial cells through IGF2-IGF2R signaling increased cholangiocellular phenotype in hepatocellular carcinoma
    Article Snippet: Polyvinyl difluorid membranes (Merck Millipore, Darmstadt, Germany) were used to transfer the proteins. .. Next, the membranes were incubated with the primary antibodies including anti-SOX2 (1:500, Affinit), anti-NANOG (1:500, Affinit), anti-OCT3 (1:1500, Afinit), anti-AKT (1:1000, CST), anti-p-AKT (1:1000, CST), anti-IGF2R (1:1000, Proteintech), anti-p-p38 MAPK (1:1000, CST), anti-p38 MAPK (1:1000, Affinit), anti-GAPDH (1:5000, Bioworld) at 4 °C for overnight, which followed by incubation with secondary antibody for 1.5 h. The membranes were visualized using enhanced chemiluminescence detection reagents (GE HealthCare, Chicago, IL, US). ..

    Knockdown:

    Article Title: Secreted Clever-1 Modulates T Cell Responses and Impacts Cancer Immunotherapy Efficacy
    Article Snippet: .. Anti-TFRC (NBP2-34602; Novus) and anti-IGF2R (20253-1-AP; Proteintech, RRID:AB_10859779) were used to detect knockdown efficiency. .. The cells were analysed with LSRFortessa (BD, RRID:SCR_018655) and data processed with FlowJo software v.10.7.1 (TreeStar, RRID:SCR_008520).

    Article Title: Secreted Clever-1 modulates T cell responses and impacts cancer immunotherapy efficacy
    Article Snippet: .. Anti-TFRC (NBP2-34602; Novus) and anti-IGF2R (20253-1-AP; Proteintech) were used to detect knockdown efficiency. .. The cells were analysed with LSRFortessa (BD) and data processed with FlowJo software v.10.7.1 (TreeStar).



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    (A) Western blotting for CREG1 was performed using the serum (1 µL) of wild type (WT) and Tg mice. (B-C) The expression of CREG1 in the soleus muscles of WT and Tg mice was examined using RT-PCR and western blot analysis. Relative levels of Creg1 mRNA (B) and CREG1 protein (C) in the soleus muscles. (D) Relative levels of insulin-like growth factor 2 receptor <t>(IGF2R),</t> phosphorylated Akt Ser 473 (pAkt), total Akt (Akt), phosphorylated mechanistic target of rapamycin (pmTOR) Ser 2448 and total mTOR (mTOR) in the soleus muscles. Data were normalized to those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (E) Western blotting for IGF2R, pAkt, Akt, pmTOR, mTOR and GAPDH in the soleus muscles. Representative images are shown. Data are presented as mean ± SEM; n = 4–6 per group. A student's t -test was performed; * P < 0.05, versus WT.
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    Image Search Results


    (A) Western blotting for CREG1 was performed using the serum (1 µL) of wild type (WT) and Tg mice. (B-C) The expression of CREG1 in the soleus muscles of WT and Tg mice was examined using RT-PCR and western blot analysis. Relative levels of Creg1 mRNA (B) and CREG1 protein (C) in the soleus muscles. (D) Relative levels of insulin-like growth factor 2 receptor (IGF2R), phosphorylated Akt Ser 473 (pAkt), total Akt (Akt), phosphorylated mechanistic target of rapamycin (pmTOR) Ser 2448 and total mTOR (mTOR) in the soleus muscles. Data were normalized to those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (E) Western blotting for IGF2R, pAkt, Akt, pmTOR, mTOR and GAPDH in the soleus muscles. Representative images are shown. Data are presented as mean ± SEM; n = 4–6 per group. A student's t -test was performed; * P < 0.05, versus WT.

    Journal: PLOS One

    Article Title: Cellular repressor of E1A-stimulated genes 1 enhances skeletal muscle performance through the stimulation of muscle differentiation and Akt-mTOR signaling pathway activation

    doi: 10.1371/journal.pone.0328485

    Figure Lengend Snippet: (A) Western blotting for CREG1 was performed using the serum (1 µL) of wild type (WT) and Tg mice. (B-C) The expression of CREG1 in the soleus muscles of WT and Tg mice was examined using RT-PCR and western blot analysis. Relative levels of Creg1 mRNA (B) and CREG1 protein (C) in the soleus muscles. (D) Relative levels of insulin-like growth factor 2 receptor (IGF2R), phosphorylated Akt Ser 473 (pAkt), total Akt (Akt), phosphorylated mechanistic target of rapamycin (pmTOR) Ser 2448 and total mTOR (mTOR) in the soleus muscles. Data were normalized to those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (E) Western blotting for IGF2R, pAkt, Akt, pmTOR, mTOR and GAPDH in the soleus muscles. Representative images are shown. Data are presented as mean ± SEM; n = 4–6 per group. A student's t -test was performed; * P < 0.05, versus WT.

    Article Snippet: The membranes were blocked for 1 h at room temperature in nonfat dry milk and then incubated overnight at 4 °C with the following antibodies: anti-CREG1 (ab233282; Abcam, Cambridge, UK), anti-Akt Ser 473 [9271; Cell Signaling Technology (CST), Danvers, MA, USA], anti-Akt (9272; CST), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2118; CST), anti- insulin-like growth factor 2 receptor (IGF2R) (14364; CST), anti- mTOR Ser 2448 (2971; CST), anti- mTOR (2972; CST), and anti-α/β-tubulin (2148; CST).

    Techniques: Western Blot, Expressing, Muscles, Reverse Transcription Polymerase Chain Reaction